![]() ![]() However, concentrates do require preparation time and validation. Given the large working dilution range, concentrates can be varied at any time to accommodate changes to laboratory practice or for multiple protocols for a particular antibody. The working dilution of concentrates can be optimized to balance cost, staining time, and quality. Concentrates are flexible, have a lower initial purchase price, and can generally be used within any staining system, both automated and manual, subject to the manufacturer’s recommendations. When selecting antibodies, there are two main options to consider a concentrated format or a pre-diluted, Ready-To-Use (RTU) format. Exposure to air and light can cause this. Antibodies over time can lose their staining intensity. Each antibody needs to be tested with your staining system. What might work well in one laboratory might not be optimal for your laboratory. There are several things to keep in mind with antibodies. Search for specific antibodies from literature, vendors, as well as your peers. This can save you a lot of headaches down the road. The counter-stain provides a contrast to the chromogen and also helps the pathologist visualize the underlying tissue structure.Ī simple, but sometimes overlooked step is to choose antibodies that work for immunohistochemistry. The blue background is a hematoxylin counter-stain that is often applied after the chromogen. The amount of staining, the staining pattern, and the location of staining (cell cytoplasm nucleus or membrane) all provide information for the diagnosing pathologist. This is a typical IHC stain where the brown precipitate indicates the presence of the target antigen – in this case, Cytokeratin 5 on a prostate biopsy. This is a process known as double staining. Stain precipitate skin#AP Red (or another red chromogen) is used mainly for skin sections where the brown DAB may be masked by brown melanin pigment.īoth DAB and AP Red are sometimes used in the same tissue section to allow the pathologist to visualize two antigens in the one slide. There are two commonly used chromogens: DAB (brown) or AP (red).ĭAB is used for most applications as it provides strong and permanent stains. The Chromogenįinally, a substrate forms an insoluble colored precipitate that can be visualized under a microscope. Multiple enzymes attached to the antibody are known as polymers, and they again produce more intense staining as there are more molecules for the chromogen to attach. Modern chromogenic detection utilizes enzymes such as Horseradish Peroxidase (HRP) that are conjugated (joined) to an antibody. The detection system builds on the secondary. It is now commonly used as multiple secondaries can bind to a single primary to amplify the staining intensity. Next, secondary antibodies bind to the primary antibody. Monoclonal antibodies have an affinity to only one epitope and tend to produce cleaner, more specific staining but are less sensitive or intense. Polyclonal antibodies have an affinity with, and bind to, multiple epitopes (or parts) or the target antigen, and as such are more prone to cross-react to non-target antigens. There are two main types of antibody, polyclonal and monoclonal. The first stage of IHC is the application of a primary antibody that binds specifically to the target antigen. Often, a pathologist will use a “panel” of multiple antigens to help fully classify a particular tumor. There are many hundreds of antigens that have been found to be diagnostically useful. Pathologists look for the presence or absence of particular antigens to assist with diagnosis. Target AntigenĪntigens are proteins that are within or on the surface of a cell. IHC is used as a diagnostic tool to assist in the diagnosis of solid tumors and cytological specimens and has been used as a mainstream diagnostic tool for almost half a century. Where H&E and Special Stains are non-specific, IHC is directed to a specific protein marker or markers. IHC has evolved to complement the Hematoxylin & Eosin (H&E) and Special Stain techniques that typically show tissue morphology (structure). IHC is used in histology to detect the presence of specific protein markers that can assist with accurate tumor classification and diagnosis. ![]()
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![]() You can set your browser to block or alert you about these cookies, but some parts These cookies are not used in a way that constitutes a “sale” of We do not allow you to opt-out of our certain cookies, as they are necessary toĮnsure the proper functioning of our website (such as prompting our cookie banner and remembering your privacyĬhoices) and/or to monitor site performance. More specifically, we use cookies and other tracking Visiting – for our advertising and marketing efforts. Use third-party cookies – which are cookies from a domain different than the domain of the website you are Those cookies are set by us and called first-party cookies. Language preference or login information. User – asks your browser to store on your device in order to remember information about you, such as your If you want to opt out of all of our lead reports and lists, please submit aĪ cookie is a small piece of data (text file) that a website – when visited by a If you do not allow these cookies you may not be This may impact theĬontent and messages you see on other websites you visit. Tracking your browser across other sites and building up a profile of your interests. Social media cookies are set by a range of social media services that we haveĪdded to the site to enable you to share our content with your friends and networks. If you do not allow these cookies, you will experience less targeted advertising. They do not store directly personal information, but are based on uniquely identifying your browser and May be used by those companies to build a profile of your interests and show you relevant adverts on other Targeting cookies may be set through our site by our advertising partners. This may affect our ability to personalize ads according to your preferences. Therefore we would not be able to track your activity through the To take that as a valid request to opt-out. If you have enabled privacy controls on your browser (such as a plugin), we have Additionally, you may contact our legalĭepartment for further clarification about your rights as a California consumer by using this Exercise My Will not hand over your personal information to any third parties. If you opt out we will not be able to offer you personalised ads and You may exercise your right to opt out of the sale of personal Personalize your experience with targeted ads. These cookies collect information for analytics and to Sale of your personal information to third parties. Under the California Consumer Privacy Act, you have the right to opt-out of the Doing the basics is required for responsible network administration.” “Vulnerabilities will continue to be found. ![]() Jack ransomwhere Patch#“Focusing on a single exploit, especially one that has a solution through a patch that was issued years ago, is really shortsighted,” he said. ![]() Digital adversaries will constantly change their methods of attack, he said, so companies and governments need to be proactive if they want to keep themselves safe. ![]() Still, NSA can only do so much on its own, and the onus falls on organizations to heed the agency’s warnings, according to Joyce. Officials worked to bring attention to the patch and took “a variety of actions to secure national security systems and provide assistance to. The company issued a patch for the vulnerability more than two years ago, but because Baltimore never updated its software, the city remained susceptible to the attack.Īfter the breach that let EternalBlue loose on the public, Joyce said the agency took significant steps to ensure government and industry had the resources they needed to fend off the weapon. Jack ransomwhere software#The attack shut down email and disrupted numerous government services, and it could ultimately cost the city more than $18 million to recover.ĮternalBlue, which was stolen during a 2017 breach at NSA, exploited a previously disclosed bug in a Microsoft software package. On May 7, hackers reportedly used an NSA tool called EternalBlue to freeze thousands of the Baltimore government’s computers. “NSA shares the concerns of all the law-abiding citizens around the world about the threat posed by that criminal, malicious cyber activity, but the characterization that there’s an indefensible nation-state tool propagating ransomware is simply untrue,” Joyce said at a cybersecurity conference hosted by CrowdStrike. ![]() Rob Joyce, the NSA’s top cyber policy adviser, on Thursday rebuffed blame after one of the agency’s cyber weapons was used to hold Baltimore’s computer networks for ransom, arguing the attack would’ve been avoided if the city was more proactive with its digital hygiene. Jack ransomwhere movie#It’s unreasonable to get mad at someone for spoiling a movie that came out more than two years ago, and a National Security Agency official thinks the same logic should apply to cybersecurity exploits. ![]() ![]() ![]()
There are a couple downloadable templates available for the program, including a Dark Horse Comics template, which is the one I prefer. I’d also be a lot more obsessive compulsive than I am, because I kind of just want to turn the thing on and run with. On the one hand, I feel that if I could harness all that raw power, I’d be pretty awesome. I found it to be much more robust than Final Draft, which has its pros and cons. #Paste celtx script into word movie#Anyway, here’s an example of something I wrote in Scrivener:įinally, I got a chance to try out Movie Magic Screenwriter, favored by iFanboy’s own Paul Montgomery, who has an actual degree in screenwriting (lest he be uncovered as the blaggard we all deeply suspect him to be), and it’s like Final Draft and then some. Man, I feel like I’m coming from the wrong place here. The great thing about this program is that it’s only about $40, so theoretically, you could buy it, never write a thing, and not feel too terrible about it. The scripting parts aren’t quite as polished as Final Draft, but they can be with a little bit of legwork and programming. It only saves a tiny step, but feels a hundred times easier to have everything organized in one place. The real strength of the program, however, lies in it’s ability to organize notes and research, and present them in a double paned window, so your outlines, or research are always available to you while you’re scripting, without having to switch apps. ![]() The program basically mimics the functions of Final Draft, and the files can even be exported into Final Draft for format tweaking. It works for novels, plays, screenplays, and of course, has a template for comics designed by writer Antony Johnston. It’s basically an all in one program for writers. #Paste celtx script into word windows#Not too long ago, I learned about a specialty writing program available only for Macs (sorry Windows folks), called Scrivener. Right?” By the way, that second one is bullshit. Or there’s the mental justification route I took, where I said, “Well, if you’re gonna spend that kind of money, it will force you to write. One, you’d better be serious to drop that kind of coinage. I think the obvious drawback to the program is that it’s not cheap. Brian Michael Bendis uses this program to write his scripts, and that might have been where I got the idea. Once you get used to it, and you’re doing the things you want to do with it, it works very well. They’re on 8 now), I sort of made up my own format, but really like how easily I can switch between characters and dialog, since I could spend more time writing, and less time highlighting and formatting. The current version will run you around $250 cheaper if you know someone with a valid school ID (You didn’t hear it from me!). With only one completed screenplay to my name (which has aged quite a bit since being written), the money spent on the program would have been wasted had I not found a way to repurpose it. Here’s what a Word script looks like.įor a while, I fancied myself a burgeoning screenwriter, so I bought Final Draft. But it certainly did the job, and lots of pros use it, to be sure. In the end, I only ever wrote one script in Word, because it required too much tabbing, spacing, bolding, and capitalizing for my tastes, and would slow me down, as I’m an impatient, yet format obsessive. I might have to pick up a CD-Rom from that “try my product” guy, now that I think about it. In fact, it’s such a robust program that I don’t really know what to do with it other than write paragraphs of text, and change the fonts therein. People with much better computer skills, and more importantly, patience, are even able to make templates and macros for comic book scripting to make it work like a specialized program. I used it because I had it, and it seemed obvious. Like many people, the first thing I ever used was Microsoft Word. This might be a little late for the folks interested in participating in the Sequentially Ever After Contest, but then, there’s still time to submit a script, and that is, of course, an acceptable entry. If you want to see some examples of comic book scripts, let me google that for you. Regardless, this article is not strictly about format, rather about the programs you can use to write scripts. If the writer is communicating what he or she needs to communicate to the artist, then it works. #Paste celtx script into word tv#Unlike TV and film scripts, the formats of which are standardized by their industries and many textbooks, comic book scripts are more of a “do what works” sort of deal. As has been said many times, by people far more experienced than I, there is no singular format for writing comics. #Paste celtx script into word how to#Here at iFanboy, we get questions, and I’ve seen plenty regarding how to write comic scripts. ![]() ![]() They have done all that they promised and I couldn't be happier. #Direct mail postcard examples free#The success of my campaign is up to me with proper follow up and my own due diligence, I will not place that on the PostcardMania Professionals as some have chosen to do. Direct Mail Postcard Samples Direct Mail, Fulfillment Services, Mailing List CALL NOW (800) 961-4877 SINCE 1978 Postcard Direct Mail Samples Postcard Mailing Tips Get a free quote today and a jump start on your next postcard mailing today Call (800) 961-4877 from Mon to Fri, 8 am to 7 pm PST. Direct mail advertisement design ideas for solar panel installation and servicing business. Solar Postcard example and Canva design template for marketing solar power business. For example, if youre gearing up for the holiday rush, youll want to plan ahead so your. Solar Direct Mail Postcard Templates & Examples. Every time I reached out to any of my pros, they responded very fast and helped me with ALL of my questions and needs. Consider these direct-mail postcard marketing statistics. ![]() One thing that most people love is to be treated like an individual, not just a number. These dental postcard marketing samples are proven to. I look forward to many campaigns with PostcardMania and would recommend them to ALL of my other business associates and friends. Find out how other dentists just like you are creating big-time marketing results for their practices. #Direct mail postcard examples driver#They have a nice group of people there and they are all so friendly and professional. To highlight the driver assist feature on their Yeti model, Skoda sent out marketing postcards with an image of a couple of. I am happy with everything about this company and I even went to visit the site and was warmly welcomed and shown around. Example of an effective frontside layout Headline: Use a compelling, active-voice statement of no more than 7 words (like an email subject line) Clear &. ![]() From Cortni, Derek, Rachel and Emily, everyone has really helped me to launch my campaign. Call (800) 961-4877 or click here to email us. ![]() From start to finish, I have had the most patient, understandable, quick in response to help crew I could ever have imagined having. Check out our church samples Contact our marketing experts if you have any questions about our nonprofit mailing services and nonprofit printing services or if you need any assistance with marketing your business/organization. ![]() ![]() ![]() ![]() At the hub is where we can find vendors located around the corners, but aren’t handed to the player. If at any point in the game the player reaches 0 HP they will die and respawn back at the hub, The Hall of Reincarnation. There is a level select, but the player must clear a level entirely before moving on. There are roughly 30 familiars in the game and will randomly spawn as Elle progresses down floor by floor. Using the RT once a second Familiar has been defeated allows the player to switch skills. Being able to teleport can remove Elle from fatal damage since the enemies can cast attacks surrounding the player. Crown also gave us the power to be able to teleport (Blink) a limited number of times before having to recharge. Using a Familiars’ skill will drain our Mana points, but refills fully after clearing a room of all the enemies. The Familiars are like mini bosses and once defeated can then be equipped to use. Each grid, or space, will matter as we unlock skills by defeating Familiars or find a new weapon with a different attack pattern. Elle can attack to advance time or click the Right stick to skip a turn. So, the game essentially works off of grids in each room and time only moves when Elle moves. #Crown trick gamepass full#The perspective is isometric meaning it’s in full 3D but does have a fixed camera. During the tutorial we come across our first chest with a spear inside and are quickly taught by Crown that traps and enemies move only when Elle is moving to a new space. The tutorial is the only part of the game where certain items will spawn consistently and are set up to help teach combat to the player. After hearing Crown’s opening story filled with information, they journey off together. There are other creatures lurking about that wish to harm her. Elle is not alone and Crown explains that she is trapped in a Dream Realm, also known as, the Nightmare Realm. Crown is a snippy character that likes to push others away and typically can come off as condescending towards humans for being ‘primitive’. Her name is Elle, a young girl around the age of 8 to 12, and seems to be gifted with harnessing magic that was imbued upon her by our Crown friend. His name is simply put, Crown, and informs our playable character of where she is. As we gain control, we explore a locked room with a sentient golden crown sitting in a chair. The story is delivered by text pop ups and there is no narration for any of the characters. Let’s talk about the story before I delve into the combat system. The goal of surviving each floor as it progressively gets harder is not so easy as the player first begins, but feels just as rewarding as any gamer would want. We play as a young girl trapped in the Nightmare Realm fighting from room to room against randomly generated enemies. #Crown trick gamepass software#This game was developed by NExT Studios and published by Team17 Software and truly does outshine some of the other games I’ve reviewed more recently. It plays as a strategy, turn based game with rogue-like features. Crown Trick is a delightful indie title that debuted on Xbox on September 7 th and also released onto Game Pass day one. ![]() ![]() ![]() Think and experiment with different vehicle settings to find the ideal "setup" that will allow you to drift in the best possible way. This is not the case of Burnout Drift, which offers these options, so you can enjoy a unique ride to your liking. Do you wish to adjust the engine power, braking ratio or steering sensitivity? Each pro will has his own style, but most games will not allow you to complexly modify the cars behavior. ![]() When you visit the garage, you will find out that you can do virtually anything with your car. You can show off on the track how you want, but what is really behind it? Great effort of mechanics and ideal car setup for a unique driving experience. #Burnout drift 1 apkTry Burnout Drift 3 : Seaport Max and Pick your best Friv 1 game in the. Download: Burnout Drift APK (Game) - BurnoutDrift APK - Latest Version: 3 - Updated: 2022 - BoneCracker Games - Free. Anyone can be a legend in the Burnout Drift Online. Burnout Drift 3 : Seaport Max is one of the amazing free games in. The exit will lead to junctions where traffic can strike at any moment. Try to score the most points for drifting. A freeway congested with dangerous traffic that forces speedy drivers to weave in and out to avoid triggering a pile-up. Puede descargar Burnout Drift 2 y los mejores juegos de Steam con GameLoop para jugar en la PC.ĭrift crazily through turns at incredible speeds and show others how it is done. Burnout Drift: Online is the multiplayer edition of the Burnout Drift franchise where you try to score as many points as possible by drifting around within the time limit. Test your wheels on the tracks There are three excellent tracks to zoom your latest set of wheels around in Burnout Drift. You can buy these powerhouse vehicles when you earn enough. Burnout Drift 2, es un popular juego de Steam desarrollado por BoneCracker Games. The first car you start with is a Ford Mustang, but there are drifting classics like the Nissan 350z to pick-ups and supercars like the Bugatti Veyron. ![]() ![]() ![]()
Search for the sub-heading labelled "Skin" and make sure to select only the 'Reload skin when confirming changes in Preferences' from the four available options. ![]() Click the FM logo at the top right-hand corner and hit "Preferences".Ĩ. You'll see a folder path for this, which will be: Your PC > Documents > Sports Interactive > Football Manager 2022 > Graphics > Logos / Kits (depending on which you created).ħ. Open the pack you've downloaded, and extract the files so that they're saved in the above folder. Whichever you create, open this folder.Ħ. This overview of free agents provides a list of those players who still played in the National League during the last season and are currently without a. If you are installing new kits, then you need to make a new folder called 'Kits'. If you are installing new logos, you need to make a new folder called 'Logos'. Inside of this 'Football Manager 2022' folder is where you'll need to create a new folder called 'Graphics' (without the quotation marks). Here you'll find another folder called 'Football Manager 2022'. VICTOR ANICHEBE ROSS WALLACE MARTIN OLSSON WILFRIED BONY HATEM BEN ARFA Hednesford make non league legend manager and ex-Premier League star player/coach. If you've already played a bit of FM22, the game will have created a folder in your Documents labelled 'Sports Interactive'. This contains thousands of logos and kits respectively.ģ. SortItOutSI tends to have great offerings, such as this TCM logos megapack and kits pack. Download the logo or kits pack of your choice. Click on players > player search and then. This will help you extract the many individual files that make up the logo pack.Ģ. To see a list of free agents known to you or your staff, all you need to do is go to the scouting tab. Football manager 2021 free agents lower league install#Firstly, install WinRAR or a similar program that's capable of opening up zipped files. Here are the simple steps to add logos to your FM22 game:ġ. Football manager 2021 free agents lower league how to#Watch on YouTube Football Managers 22 new features preview How to add logos and kit packs in Football Manager 2022 Today we look at the best free players to sign in your Non League Football Manager save gameFootball Manager 2021 Top X Playlist. ![]() ![]() If it is plugged in and spraying the pins with cleaner doesn't help then you probably need a new coolant tank. Check that the warning goes away before you put the coolant tank back in and screw it down. If it is plugged in then spray the pins with some electrical contact cleaner and plug it back in. Take the one screw out for the coolant tank, then lift the coolant tank out of the tab retainers, and check that it is plugged in. There is a low level sensor in the bottom of the coolant tank. The CEL went away after a couple days so i wasn't able to read it, and i do not have a Ross-tech to read what has been stored.Īny help is greatly appreciated, thank you! This, and a random CEL from last week, have me wondering. I was wondering why it would take this long. Lately, i've seen it only get to the 1/4 mark after about 10 minutes and may not get up near the 1/2 mark until i'm almost home from my 30 minute commute. Typically the car gets to the middle on the engine temp gauge in about 10 minutes. Is there some sort of low-level sensor i can look for and possibly replace? What would cause this error other than actually having low coolant? There shouldn't be a leak anywhere as i have plenty of coolant.Īs for things that may have changed recently - i have noticed that on some days it takes quite a while for my engine to get warm. Needless to say, i'm a little scared to drive it in the event there's some sort of major failure (car runs great still, from what i can tell). So after i added the coolant, slightly above the "max" line, i started it up again and got the same warning light. Before adding it, i realized i was right in the middle, so i was unsure why it was beeping at me but i added more anyway. ![]() The next morning i started the car and of course the light was still on, beeping at me, so i added coolant to the tank. Drove 5 more minutes to my house and shut the car off. Car temperature was right in the middle where it usually is, and I've never seen it get above this point. This popped on the other day while driving for maybe 10 minutes. I'm wondering if anyone can troubleshoot an issue with my coolant light. Cylinder head gasket leaking combustion into cooling systemĪn overheated engine usually ruin the thermostat, so you should always replace the thermostat after an overheating incident.Another month, another issue.LOL. ![]()
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Main features: - Support for more than 5. This article is about the Pokmon that were introduced in Generation V. ![]() #POKEMMO MODS GEN 5 FOLLOW MOD#Might be able to do that with this mod to get the 'updated' ones + sparkly. My personal favorite is to use the sparkly one + a normal one combined, so they sparkle but don't bounce. #POKEMMO MODS GEN 5 FOLLOW ZIP FILE#zip file download for MacOS and fixed some things at the mod, btw. ![]() If you wish to report a specific issue, please open a thread in the Wiki Feedback forum. Bearminator just added a poll, if the jumping animation should be removed or not.Classic sprites don't got this animation, so I'm wondering what you think. We are currently in the process of migrating these articles, so please excuse any formatting or credit issues that may be present. #POKEMMO MODS GEN 5 FOLLOW SERIES#Whether you're interested in playing online with others, catching brand new Pokemon, or even replaying a classic Pokemon game with increased difficulty, these modders and indie developers have definitely got you covered. A total of 5 main series Pokmon games were released for this console, bringing us the third generation of Pokmon. Maybe some side-by-side pictures of how they were updated because there's like 5 different follower sprite mods. Most of these articles were previously on our wiki. If you happen to be one of them, you might want to consider checking out one of the many excellent fan-made Pokemon games in order to stave off the boredom while you wait for November to roll around. Reflect now remains in effect for five turns and will double the Defense of. Updated Jby Tom Bowen: With no more DLC expansions for Pokemon Sword & Shield currently planned and the Diamond & Pearl remakes and Pokemon Legends: Arceus still many months away, a lot of Pokemon fans currently find themselves with no new games to play. If the opponent has Reflect, this lowers the users confusion damage. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators. So if you're looking to spice it up and see the Pokemon franchise in a whole new light, one of these Pokemon fan-games might just scratch that itch. RELATED: The Best Pokemon Professors Ranked By Teaching AbilityĪlso, you know this to be true: the official games can get kinda boring sometimes. Of course, some Pokemon fan games float to the surface more than the others, but the ones that due are true gems. The proof of that is in the endless pool of fan-made games out there. form Gloom is the pokemon whish has two types ( Grass and Poison) from the 1 generation. It just so happens that a lot of Pokemon fans are aspiring game designers, and Pokemon is just as much of a creative outlet as much as it is a game franchise. Pixelmon (Minecraft Mod) The Pixelmon TCG has come a long way. ![]() ![]() We will be creating separate posts for prototyping responsive designs and testing them in a live environment or in an emulator and for other informative resources that are so extensive that they need their own page discussing why they are required for responsive web design. ![]() If the information listed here is not enough, then use your search engine of choice and do some searching, some terms you could search for: You can sit down for hours reading the posts and the books, digest the information and then possibly start out and create your own responsive design. The lists above are short, but the content they link to is immense. Responsive Layouts, Responsively Wireframed Responsive Web Design Sketch Sheets or download the files here #Templates for responsive site designer seriesRedesign The Web (The Smashing Book 3) by Smashing eBook Series Responsive Design by Smashing eBook Series Responsive Web Design Books You Can Buy (We Did): In the process of moving to a responsive design for your site, the. #Templates for responsive site designer how toResponsive Web Design: What It Is and How To Use It By Kayla Knight for SmashingMagazine Get your content ready to go anywhere because its going to go everywhere. The original post about responsive web design from Ethan MarcotteĪ Beginner's Guide to Responsive Web Design by Nick Pettit from Treehouse More than a WordPress theme, it is an alternative website building platform. It comes with advanced features like drag and drop editor, visual editing, custom CSS control, design option galore, responsive editing, global element and style, inline text editing, so on and so forth. Here we have compiled some of the responsive web design resources that we used to understand responsive web design: Divi is the ultimate responsive website template that you can use. Having personally over 10 years experience of developing websites from scratch made it easier to understand the concept and idea behind responsive web design. are required.Īs we started to build responsive layouts and templates it obviously didn't take that long to get the coding part working as it should, but understanding what we were actually doing took longer. When starting out or learning to develop a responsive web design a lot of resources including demos, ideas, information etc. ![]() |
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